Cells in tissue culture can be maintained using Minimal Essential Medium (MEM), a synthetic cell culture medium created by Harry Eagle and initially published in Science in 1959. pH ranges from 7.0 to 7.4.
includes non-essential amino acids, 1500 mg/L sodium bicarbonate, 1 mM sodium pyruvate, 2 mM L-glutamine, and Earle's Balanced Salt Solution.